Extended Data Fig. 7: Splitting at N67 (DdCBE-TOD6_N67) achieves efficient and precise editing.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a, Architectures of split DdCBE_S, DdCBE, DdCBE-TOD6_S, and DdCBE-TOD6 at the C-terminal of N29 or N94 for E. coli-based editing assays. b, The editing efficiency of N29 and N94 splits in E. coli. Base editors were induced with 0.2 g/L arabinose for 1 h. c, The editing efficiency of N-half and C-half components of the N29 and N94 splits in E. coli. DdCBE and DdCBE-TOD6 share the same C-half split. Base editors were induced with 0.2 g/L arabinose for 1 h. d, Schematic representation of DdCBE_N29 and DdCBE-TOD6_N29 for mtDNA editing. e, f, The mtDNA editing efficiencies of DdCBE_N29 and DdCBE-TOD6_N29 at the MT-CYB (e) and MT-TS2 (f) site in HEK293T cells. The transfection duration was 3 days. g, h, The editing efficiency of DdCBE-TOD6_N67 split and its N-half and C-half components in E. coli. DdCBE-TOD6 variants were induced with 0.2 g/L arabinose for 1 h (g) or 3 h (h). Data are presented as mean ± SD from n = 3 independent experiments. Source data are provided as a Source Data file.
