Extended Data Fig. 9: DdCBE-TOD6-derived constructs achieve single-nucleotide precision editing at non-CC context positions.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a–e, mtDNA editing efficiencies of DdCBE_N94 and DdCBE-TOD6-derived constructs at the MT-CO3 (a), MT-ND5 (b), MT-ND1.1 (c), MT-ND1.2 (d) and MT-ND1.3 (e) sites in HEK293T cells. Note that MT-ND5 site contains a CCCC sequence, and MT-ND1.3 site contains an ACC sequence. f–h, mtDNA editing efficiencies of DdCBE_N94 and DdCBE-TOD6-derived constructs at the mt-Nd1.1 (f), mt-Nd1.2 (g) and mt-Nd6 (h) sites in NIH/3T3 cells. Data are presented as mean ± SD from n = 3 independent experiments. The transfection duration was 3 days. i, Distribution of disease-associated mtDNA mutations across different sequence contexts. Source data are provided as a Source Data file.
