Extended Data Fig. 10: DdCBE-TODs achieve high precision mtDNA editing in human primary skin fibroblast and MEF cells.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a, Average mtDNA genome-wide C•G to T•A conversion rate induced by DdCBE-TOD6_sCUTV and DdCBE-TOD6_dNU for MT-TK.2 sites in human primary skin fibroblasts. Naturally occurring single nucleotide variations (SNVs) with heteroplasmy fraction >10% were excluded from the analysis. Data represent the mean from n = 2 independent experiments. b, m.G8369 mutation load in untreated and DdCBE-TOD6_sCUT-treated MEF clones. Each dot represents the mutation load measured from a single-cell-derived clone. Successfully edited MEF clones by DdCBE-TOD6_sCUT exhibited mutation loads ranging from 42.01% to 89.89%, with a median mutation load of 70.05%. Median with interquartile range is shown, n = 4 for untreated, n = 53 for DdCBE-TOD6_sCUT. c, Heat map showing C•G-to-T•A conversion frequencies for 4 untreated clones and 4 clones with the highest mutation loads after DdCBE-TOD6_sCUT treatment, as depicted in panel (b). Shown are individual values. Source data are provided as a Source Data file.
