Extended Data Fig. 1: mDdCBEs using a truncated Ddd_Ss variant demonstrate significant mtDNA editing activity.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a, Architectures of DdCBE and mDdCBEs for mtDNA editing. A truncated Ddd_Ss variant (Ddd_Ss_S, denoting Ddd_Ss_Short, residues 2–114), which lacks the C-terminal SPKK-related motif, was utilized to construct mDdCBEs. MTS, mitochondrial localization sequences. b, Heat maps showing C•G-to-T•A conversion frequencies for DdCBE and mDdCBEs at the MT-ND6 site in mtDNA of HEK293T cells. Shown are individual values from n = 3 independent experiments. c, Indel frequencies of DdCBE and mDdCBEs at the MT-ND6 site in mtDNA. Shown are mean ± SD from n = 3 independent experiments. The transfection duration was 3 days. Source data are provided as a Source Data file.
