Fig. 4: Cryo-EM structures of ICMTs and apical SPMTs.
From: Atomic models of the Toxoplasma cell invasion machinery
a, Representative cryo-EM image showing a pair of ICMTs within the conoid. b, Example of an ICMT pair dislodged from the conoid, revealing fibrous densities on their sides. c, Selected 2D class averages of ICMT particles showing different views. d, Cross-sectional view of the cryo-EM structures of ICMT-1 and ICMT-2, with associated proteins. The light-purple density in the background is our single-particle 3D reconstruction of the ICMT pair, obtained before refining each ICMT individually. e, Tilted view of ICMT-2 showing that TLAP3 spans laterally across 11 of the 13 protofilaments, while TLAP4 binds specifically at the microtubule seam. TLAP3 and TLAP4 in ICMT-1 and apical SPMT exhibit similar appearances. f,g, Costaining of TLAP3 (f) and TLAP4 (g) with tubulin markers using mouse anti-Ty and anti-mouse IgG Alexa Fluor 488 (green) and rabbit anti-Tubulin and anti-rabbit IgG Alexa Fluor 568 (red) for U-ExM. h,i, Longitudinal views of ICMT-1 showing ICMAP1 (h) and ICMAP2 (i), respectively. j, Longitudinal view of ICMT-2 showing MAP densities (green), with some confidently assigned to ICMAP4 (medium orchid).
