Table 1 Proteins identified by cryo-EM in this study, organized by complex

From: Atomic models of the Toxoplasma cell invasion machinery

Number	Protein name^a	Gene ID TGME49_	Number of residues	Number of copies per asymmetric unit^b	M/S ranking^c	Location		Growth phenotype^d		References
Number	Protein name^a	Gene ID TGME49_	Number of residues	Number of copies per asymmetric unit^b	M/S ranking^c	hyperLOPIT (Markov chain Monte Carlo)	This study	CRISPR score	Experimental	References
1	CF1	222350	410	2	77	Apical 1	CF	−1.31	Normal	²⁵, this study
2	CF2	258090	954	16^e	18	Apical 1	CF	−1.34	Normal	²⁵
3	CF3	255895	556	1	81	Apical 1	CF	0.23	N/A	²⁵
4	CF4	246720	502	1	59	Apical 2	CF	0.24	Normal	²⁵
5	CF5	297180	871	1	74	Apical 1	CF	−1.52	Normal	This study
6	CF6	245640	416	4	180	Apical 1	CF	−0.33	N/A	²⁵
7	CIP3	225020	1,010	1	16	Apical 1	CF	−2.78	Modest	²⁵, this study
8	CPH1	266630	582	4	43	Apical 1	CF	−4.16	Essential	²⁵
9	DCX	256030	256	4	191	Apical 1	CF	−5.03	Strong	^37,38
10	PRP	291880	783	2	221	Apical 1	CF	1.77	Normal	²⁷
11	SAS6L	301420	263	6	218	Apical 1	CF	−1.62	Modest	⁴⁰, this study
12	SPM1	263520	220	1^f	220	Tubulin cytoskeleton	CF, ICMT, SPMT	1.21	Normal	^21,22,46
13	ICMAP1	239300	1,232	2	6	Apical 2	ICMT	−0.74	Normal	^10,49
14	ICMAP2	224700	1,678	2	22	Apical 2	ICMT	−1.56	Strong	¹⁰
15	ICMAP4	225340	2,041	1	44	N/A	ICMT	0.73	N/A	This study
/	SPM1	263520	351	11^f	220	Tubulin cytoskeleton	CF, ICMT, SPMT	1.21	Normal	^21,22,46
16	TLAP2	232130	446	2	350	Tubulin cytoskeleton	SPMT	−0.82	Normal	⁴⁷
17	TLAP3 (AC5)	235380	583	1	80	Apical 1	ICMT, apical SPMT	1.44	N/A	⁴⁷
18	TLAP4	201760	336	1	514	Apical 1	ICMT, apical SPMT	0.54	N/A	⁴⁷
19	TrxL1	232410	220	11	346	Tubulin cytoskeleton	ICMT, SPMT	0.99	Normal	^21,46
20	TrxL2	225790	189	1	567	Tubulin cytoskeleton	ICMT, SPMT	1.98	Normal	^21,46
21	CAM1	246930	179	1	311	N/A	PCR-P2	1.09	Normal	⁴³
22	CAM4	249240	149	1	771	Cytosol	PCR-P2	−5.28	N/A	This study
23	CGP	240380	4,956	1	97	PM - peripheral 2	PCR-P2	−3.85	Strong	⁵¹
24	FLM1	271780	2,777	1	116	N/A	PCR-P2	−2.75	Essential	This study
25	FLM2	285990	1,192	1	413	N/A	PCR-P2	−2.97	Essential	This study
26	FRM1	206430	5,009	2	89	N/A	PCR-P2	−3.24	Essential	^6,13,52, this study
27	ICAP16	202120	1,322	4	72	PM - peripheral 2	PCR-P2	−2.1	Normal	⁴²
28	MLC4	294390	172	1	951	N/A	PCR-P2	0.38	N/A	⁵⁶
29	MyoL	291020	2,484	1	58	N/A	PCR-P2	−1.83	Strong	⁵⁵, this study
30	PCR4	201220	603	2	278	N/A	PCR-P2	−5.4	Essential	⁶
31	PCR5	242320	1,073	2	96	Apical 2	PCR-P2	−3.14	Essential	⁶
32	PCR10	298010	2,322	1	41	N/A	PCR-P2	−2.55	Normal	This study
33	PCR11	209490	505	1	269	N/A	PCR-P2	0.49	N/A	N/A
34	PCR12	219070	2,720	2	57	N/A	PCR-P2	−2.2	N/A	N/A
35	PCR13	284620	2,333	1	70	N/A	PCR-P2	−1.02	N/A	N/A
36	PCR14	311880	728	2	84	N/A	PCR-P2	2.34	N/A	N/A
37	PCR15	232560	1,822	1	276	N/A	PCR-P2	−0.4	N/A	N/A
38	AKMT2	292170	1,599	1	131	N/A	PCR-P2	−4.83	Essential	This study
39	SEC23	291680	791	2	210	Nucleus - non-chromatin	PCR-P2, ER–Golgi interface	−5.46	Normal	This study
40	SEC24	277000	1,019	2	396	Nucleus - chromatin	PCR-P2, ER–Golgi interface	−4.58	Essential	This study

^aProtein names highlighted in dark orange are given in this study.
^bFor CF, ICMT and SPMT component proteins, the number of copies per asymmetric unit means the number of proteins per 8-nm repeat length.
^cM/S rankings are based on the total unique peptide count (Supplementary Table 1).
^dThe CRISPR (fitness) score⁴² and hyperLOPIT²⁷ data were obtained from ToxoDB⁷⁵. Genes with low scores are predicted to be essential. Experimental growth phenotypes analyzed in this study are highlighted in blue. N/A, not available.
^eThe periodicity for CF2 is 24 nm instead of 8 nm, with 16 copies of CF2 (8 homodimers) per 24-nm repeat length.
^fThe actual periodicity of SPM1 is unclear because of the sequence similarity of its internal repeats (six Mn motifs)²¹.

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Table 1 Proteins identified by cryo-EM in this study, organized by complex

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