Extended Data Fig. 3: Heterochromatin-associated proteins in germ cells.
From: Global reorganization of genome architecture at the transition to gametogenesis
a, Representative IF image showing downregulation of Hp1 α. Scale bar: 10 µm. b, Comparative gene expression of of Hp1 α(Cbx5), HP1β (Cbx1), HP1Ƴ (Cbx3) using published scRNA-seq data26. c, ATRX is retained in the mitotic chromosomes in PGCs. Scale bar: 10 µm. d, Representative cropped transmission electronic microscope image highlighting nuclear pores and perinuclear heterochromatin. Scale bar: 100 nm. e, Representative IF image of LAMIN A/C. Scale bar: 10 µm. f, Representative IF image of LBR. Yellow arrowheads indicate germ cells. White arrowheads indicate surrounding somatic cells. Scale bar: 10 µm. g, Representative IF image of H3K9me3 after Chaetocin treatment. Scale bar: 5 µm. h, Gonads were cultured with Chaetocin for 24 hours. Scale bar: 5 µm. Measurement of H3K9me3 intensity. Mean value of each nucleus was calculated. n represents the cell number analyzed. P values were calculated by 2 tailed Mann-Whitney U test. Box plots were plotted using Tukey’s method. The median and the first and third quartiles are indicated. The whiskers represent the furthest value within 1.5 × interquartile range (IQR). (a, c, d, e, f, g) Representative of 3 independent experiments (3 embryos from independent litters).
