Extended Data Fig. 4: Flowchart of cryo-EM data processing for the human MPU complex.

(a) Representative micrograph of the cryo-EM data collection from 2,191 micrographs. (b) Workflow of the cryo-EM data processing. Details are described in the Methods section. (c) Representative micrograph of the 2D classification generated from the cryo-EM particles of human MPU complex. (d) Particle angular distribution for the map of the human MPU complex. (e) Gold-standard Fourier shell correlation (FSC) curve for the refined map of the human MPU complex.