Supplementary Figure 1: Effect of diamide or oxamate treatment on subcellular pH. | Nature Protocols

Supplementary Figure 1: Effect of diamide or oxamate treatment on subcellular pH.

From: Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors

Supplementary Figure 1

(a and b) Fluorescence imaging (top) and fluorescence changes (bottom, n = 6 cells) in HeLa cells simultaneously expressing cytosol-localized iNapc and cytosol-localized pHRFP in response to 200 µM diamide (a) or 2 mM oxamate (b). (c and d) Fluorescence imaging (top) and fluorescence changes (bottom, n = 6 cells) in HeLa cells simultaneously expressing mitochondria-localized iNapc, nuclear-localized iNapc, and mitochondrial-localized pHRFP in response to 200 µM diamide (c) or 2 mM oxamate (d). Data are the mean ± s.d. All p values were obtained using unpaired two-tailed Student’s t test. *p < 0.05, ***p < 0.001. Scale bars, 10 µm.

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