Supplementary Figure 4: Flow cytometry analysis of the pH state in resting and activated mouse macrophages. | Nature Protocols

Supplementary Figure 4: Flow cytometry analysis of the pH state in resting and activated mouse macrophages.

From: Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors

Supplementary Figure 4

(a) Cytosolic or mitochondrial pH detection in resting or activated RAW264.7 mouse macrophages by flow cytometry. (b) Quantitative data for cytosolic or mitochondrial pH sensor fluorescence were obtained from three or more independent detections by flow cytometry. Data are the mean ± s.e.m. All p values were obtained using unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

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