Supplementary Figure 2: Comparison of extracted band intensities from Supplementary Fig. 1. | Nature Protocols

Supplementary Figure 2: Comparison of extracted band intensities from Supplementary Fig. 1.

From: Structural interpretation of DNA–protein hydroxyl-radical footprinting experiments with high resolution using HYDROID

Supplementary Figure 2

Solutions with no constraint options employed during fitting manifest unphysical fluctuations due to overfitting (nearby bands should have similar widths) in the region where the bands are located close together. The use of Lorentzians versus Gaussians provides slightly altered results in the low and high regions of the cleavage frequency values. Zero on the x-axis marks the nucleotide positioned on the central dyad symmetry axis of nucleosome.

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