Supplementary Figure 2: Outline of the gating strategy implemented for sorting HEK293FT single cells. | Nature Protocols

Supplementary Figure 2: Outline of the gating strategy implemented for sorting HEK293FT single cells.

From: Small-seq for single-cell small-RNA sequencing

Supplementary Figure 2: Outline of the gating strategy implemented for sorting HEK293FT single cells.

Gates for selecting single cells are shown in plots for Side Scatter Area (SSC-A, logarithmic) against Forward Scatter Area (FSC-A) (a), Forward Scatter Width (FSC-W) against Forward Scatter Area (FSC-A) (b), and Side Scatter Width (SSC-W) against Side Scatter Area (SSC-A, logarithmic) (c). Propidium iodide was applied to stain for dead cells (d).

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