Supplementary Figure 2: Gating strategy.

Non-transfected EPSCs (DR25) were used as negative control. In all sorting experiments, cell population was initially identified on a FSC/SSC plot, using a polygon gate to separate distinct cell population from debris and feeder (over 85%). The boundary between positive and negative was determined by negative control. And negative population in control was over 99.5%. Using these gates, the sample, DR25-EPSCs transfected with an mCherry fluorescence reporter construct was sorted (76.5% mCherry positive) and plated. a. Negative control, untransfected DR25-EPSCs. b. Sample, DR25-EPSCs transfected with PBCAGmCherry.