Supplementary Figure 1: Measuring the expansion factor manually.
From: A practical guide to optimization in X10 expansion microscopy
(a) The same images as shown in Fig. 7 for determining the expansion factor via the script provided with this manuscript are used here for manual determination of the expansion factor. 10 distance measurements were taken, using the respective tool in ImageJ, between landmark points that are easily identifiable in the pre- and post-expansion image. The lines for distance measurements were separately drawn manually in both images. Corresponding lines are numbered in both images. Scale bar: 20 µm. (b) Results of the same 10 manual distance measurements, together with the ratio of the measurement after expansion over the measurement before expansion. The average ratio is indicated below, and serves as the expansion factor for this image: 7.98. The manual measurement is consistent with the automatic measurement via the script (expansion factor: 8.0, see Fig. 7), but is considerably more time-consuming.
