Supplementary Figure 5: Gating strategy for the identification of pMHC-multimer-positive CD8+ T cells.
From: Induction of neoantigen-reactive T cells from healthy donors
Lymphocytes were identified, and doublets and dead cells were excluded with the help of forward (FSC) and side scatter (SSC) gates and live/dead fixable near-IR dead cell staining. From the live cell gate, CD8+ T cells were gated and pMHC-multimer-reactive cells were identified as double positive for PE- and APC-conjugated pMHC multimers.
