Supplementary Fig. 5.: Characterization of adiponectin (19–107) (C36Acm)—Payne Lab, University of Sydney. | Nature Protocols

Supplementary Fig. 5.: Characterization of adiponectin (19–107) (C36Acm)—Payne Lab, University of Sydney.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 5.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Adiponectin (19–107) (C36Acm); Rt 4.22 min (0 to 50% B over 5 min, λ = 214 nm). Calculated Mass [M+5H]5+: 1759.1, [M+6H]6+: 1466.1, [M+7H]7+: 1256.8, [M+8H]8+: 1099.8, [M+9H]9+: 977.7, [M+10H]10+: 880.1, [M+11H]11+: 800.1; Mass Found (ESI+): 1758.7 [M+5H]5+, 1466.1 [M+6H]6+, 1256.8 [M+7H]7+, 1099.9 [M+8H]8+, 977.8 [M+9H]9+, 880.2 [M+10H]10+, 800.2 [M+11H]11+.

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