Supplementary Fig. 7.: Characterization of purified adiponectin (19–107)—Payne Lab, University of Sydney.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Adiponectin (19–107); R_t 4.48 min (0 to 50% B over 5 min, λ = 214 nm). Calculated Mass [M+5H]⁵⁺: 1745.3, [M+6H]⁶⁺: 1454.6, [M+7H]⁷⁺: 1246.9, [M+8H]⁸⁺: 1091.2, [M+9H]⁹⁺: 970.1, [M+10H]¹⁰⁺: 873.2, [M+11H]¹¹⁺: 793.9, [M+12H]¹²⁺: 727.8; Mass Found (ESI+): 1745.1 [M+5H]⁵⁺, 1454.2 [M+6H]⁶⁺, 1246.8 [M+7H]⁷⁺, 1091.1 [M+8H]⁸⁺, 970.0 [M+9H]⁹⁺, 873.1[M+10H]¹⁰⁺, 793.8 [M+11H]¹¹⁺, 727.8 [M+12H]¹²⁺.