Supplementary Fig. 11.: Acm deprotection of adiponectin (19–107) (C36Acm)—Dep. Research Chemistry, Novo Nordisk.

a) Analytical reverse-phase HPLC trace (C18 CSH) and b) ESI mass envelope of reverse-phase HPLC purified Adiponectin (19–107). Rt 2.04 min (5% B over 0.5 min followed by 5 to 60% B over 3.5 min, λ = 214 nm); Calculated Mass [M+5H]⁵⁺: 1745.3, [M+6H]⁶⁺: 1454.6, [M+7H]⁷⁺: 1246.9, [M+8H]⁸⁺: 1091.2, [M+9H]⁹⁺: 970.0, [M+10H]¹⁰⁺: 873.1, [M+11H]¹¹⁺: 793.9, [M+12H]¹²⁺: 727.8; Mass Found (ESI+): 1744.9 [M+5H]⁵⁺, 1454.4 [M+6H]⁶⁺, 1246.8 [M+7H]⁷⁺, 1091.1 [M+8H]⁸⁺, 970.0[M+9H]⁹⁺, 873.1 [M+10H]¹⁰⁺, 793.8 [M+11H]¹¹⁺, 727.7 [M+12H]¹²⁺.