Supplementary Fig. 12.: Characterization of haemathrin-1 N-terminal selenoester (1–23)—Payne Lab, University of Sydney.
From: Diselenide–selenoester ligation for chemical protein synthesis
a) Analytical reverse-phase HPLC trace and B) ESI mass envelope of reverse-phase HPLC purified Haemathrin-1 selenoester (1–28). Rt 3.91 min (0 to 50% B over 5 min, λ = 214 nm); Calculated Mass [2M+3H]3+: 1856.9, [M+2H]2+: 1393.0, [M+3H]3+: 929.0, [M+4H]4+: 697.0, [M+5H]5+: 557.8, [M+6H]6+: 465.0; Mass Found (ESI+): 1856.2 [2M+3H]3+, 1392.2 [M+2H]2+, 928.5 [M+3H]3+, 696.7 [M+4H]4+, 557.5 [M+5H]5+, 464.7 [M+6H]6+.
