Supplementary Fig. 13.: Characterization of haemathrin-1 N-terminal selenoester (1–23)—Dep. Research Chemistry, Novo Nordisk.
From: Diselenide–selenoester ligation for chemical protein synthesis
a) Analytical reverse-phase HPLC trace (C18 BEH) and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-1 selenoester (1–23). Rt 2.21 min (5 to 20% B over 0.5 min followed by 20 to 40% B over 3.5 min, λ = 214 nm); Calculated Mass [M+2H]2+: 1392.7, [M+3H]3+: 928.8, [M+4H]4+: 696.8, [M+5H]5+: 557.7; Mass Found (ESI+): 1392.5 [M+2H]2+, 928.6 [M+3H]3+, 696.7 [M+4H]4+, 557.6 [M+5H]5+.
