Supplementary Fig. 14.: Characterization of haemathrin-1 C-terminal diselenide dimer (24–59)—Payne Lab, University of Sydney. | Nature Protocols

Supplementary Fig. 14.: Characterization of haemathrin-1 C-terminal diselenide dimer (24–59)—Payne Lab, University of Sydney.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 14.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-1 diselenide dimer (29–59); Rt 3.41 min (0 to 50% B over 5 min, λ = 215 nm). Calculated Mass [2M+5H]5+: 1658.3, [2M+6H]6+: 1382.1, [2M+7H]7+: 1184.8, [2M+8H]8+: 1036.8, [2M+9H]9+: 921.7, [2M+10H]10+: 829.6, [2M+11H]11+: 754.3, [2M+12H]12+: 691.5; Mass Found (ESI+); 1657.5 [2M+5H]5+, 1381.4 [2M+6H]6+, 1184.3 [2M+7H]7+, 1036.4 [2M+8H]8+, 921.3 [2M+9H]9+, 829.3 [2M+10H]10+, 754.0 [2M+11H]11+, 691.2 [2M+12H]12+.

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