Supplementary Fig. 15.: Characterization of haemathrin-2 N-terminal selenoester (1–28)—Payne Lab, University of Sydney.
From: Diselenide–selenoester ligation for chemical protein synthesis
a) Analytical reverse-phase HPLC trace and B) ESI mass envelope of reverse-phase HPLC purified Haemathrin-2 selenoester (1–28). Rt 3.91 min (0 to 50% B over 5 min, λ = 214 nm); Calculated Mass [M+2H]2+: 1701.3, [M+3H]3+: 1134.5, [M+4H]4+: 851.2, [M+5H]5+: 681.1, [M+6H]6+: 567.8, [M+7H]7+: 486.8; Mass Found (ESI+): 1701.1 [M+2H]2+, 1134.4 [M+3H]3+, 851.0 [M+4H]4+, 681.1 [M+5H]5+, 567.7 [M+6H]6+, 486.8 [M+7H]7+.
