Supplementary Fig. 17.: Characterization of haemathrin-2 C-terminal diselenide dimer (29–59)—Payne Lab, University of Sydney. | Nature Protocols

Supplementary Fig. 17.: Characterization of haemathrin-2 C-terminal diselenide dimer (29–59)—Payne Lab, University of Sydney.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 17.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-2 diselenide dimer (29–59); Rt 3.61 min (0 to 50% B over 5 min, λ = 215 nm). Calculated Mass [M+2H]2+: 1773.3, [2M+5H]5+: 1418.4, [M+3H]3+: 1182.5, [2M+7H]7+: 1013.5, [M+4H]4+: 887.2, [2M+9H]9+: 788.5, [M+5H]5+: 709.9, [2M+11H]11+: 645.3; Mass Found (ESI+); 1774.3 [M+2H]2+, 1419.4 [2M+5H]5+, 1182.9 [M+3H]3+, 1014.0 [2M+7H]7+, 887.3 [M+4H]4+, 788.7 [2M+9H]9+, 709.9 [M+5H]5+, 645.6 [2M+11H]11+.

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