Supplementary Fig. 18.: Characterization of haemathrin-2 C-terminal diselenide dimer (29–59)—Dep. Research Chemistry, Novo Nordisk. | Nature Protocols

Supplementary Fig. 18.: Characterization of haemathrin-2 C-terminal diselenide dimer (29–59)—Dep. Research Chemistry, Novo Nordisk.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 18.

a) Analytical reverse-phase HPLC trace (C18 BEH) and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-2 diselenide (29–59). Rt 2.48 min (5 to 15% B over 0.5 min followed by 15 to 35% B over 3.5 min, λ = 214 nm); Calculated Mass [2M+4H]4+: 1772.8, [2M+5H]5+: 1418.4, [2M+6H]6+: 1182.2, [2M+7H]7+: 1013.4, [2M+8H]8+: 886.9, [2M+9H]9+: 788.5, [2M+10H]10+: 709.7; Mass Found (ESI+): 1772.6 [2M+4H]4+, 1418.3 [2M+5H]5+, 1182.1 [2M+6H]6+, 1013.4 [2M+7H]7+, 887.0 [2M+8H]8+, 788.4 [2M+9H]9+, 709.8 [2M+10H]10+.

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