Supplementary Fig. 20.: Characterization of haemathrin-1 (1–59)—Payne Lab, University of Sydney. | Nature Protocols

Supplementary Fig. 20.: Characterization of haemathrin-1 (1–59)—Payne Lab, University of Sydney.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 20.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-1; Rt 3.41 min (0 to 50% B over 5 min, λ = 214 nm). Calculated Mass [M+4H]4+: 1673.5, [M+5H]5+: 1339.0, [M+6H]6+: 1116.0, [M+7H]7+: 956.7, [M+8H]8+: 837.3, [M+9H]9+: 744.3, [M+10H]10+: 670.0, [M+11H]11+: 609.2; Mass Found (ESI+): 1673.1 [M+4H]4+, 1338.8 [M+5H]5+, 1115.8 [M+6H]6+, 965.5 [M+7H]7+, 837.1 [M+8H]8+, 744.2 [M+9H]9+, 669.9 [M+10H]10+, 609.1 [M+11H]11+.

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