Supplementary Fig. 25.: Characterization of haemathrin-2 (1–59)—Payne Lab, University of Sydney. | Nature Protocols

Supplementary Fig. 25.: Characterization of haemathrin-2 (1–59)—Payne Lab, University of Sydney.

From: Diselenide–selenoester ligation for chemical protein synthesis

Supplementary Fig. 25.

a) Analytical reverse-phase HPLC trace and b) ESI mass envelope of reverse-phase HPLC purified Haemathrin-2; Rt 3.63 min (0 to 50% B over 5 min, λ = 214 nm). Calculated Mass [M+4H]4+: 1678.3, [M+5H]5+: 1342.8, [M+6H]6+: 1119.2, [M+7H]7+: 959.5, [M+8H]8+: 839.7, [M+9H]9+: 746.5, [M+10H]10+: 671.9, [M+11H]11+: 610.9; Mass Found (ESI+): 1677.9 [M+4H]4+, 1342.5 [M+5H]5+, 1118.9 [M+6H]6+, 959.3 [M+7H]7+, 839.5 [M+8H]8+, 746.3 [M+9H]9+, 671.7 [M+10H]10+, 610.8 [M+11H]11+. * Indicates artefact masses due to dimerization.

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