Supplementary Fig. 2.: Characterization of adiponectin 19–40-SePh (C36Acm)—Dep. Research Chemistry, Novo Nordisk.
From: Diselenide–selenoester ligation for chemical protein synthesis
a) Analytical reverse-phase HPLC trace (C18 BEH) and b) ESI mass envelope of reverse-phase HPLC purified Adiponectin selenoester (19-40). Rt 2.93 min (5 to 30% B over 0.5 min followed by 30 to 50% B over 3.5 min, λ = 214 nm); Calculated Mass [M+2H]2+: 1235.1, [M+3H]3+: 823.7; Mass Found (ESI+): 1234.9 [M+2H]2+, 823.6 [M+3H]3+.
