Supplementary Fig. 3.: Characterization of adiponectin C-terminal diselenide dimer (41–107)—Payne Lab, University of Sydney.

a) Analytical reverse-phase HPLC trace and B) ESI mass envelope of reverse-phase HPLC purified Adiponectin diselenide dimer (41–107); R_t 3.87 min (0 to 50% B over 5 min, λ = 214 nm). Calculated Mass [2M+7H]⁷⁺: 1875.5 [2M+8H]⁸⁺: 1641.2, [2M+9H]⁹⁺: 1459.0, [2M+10H]¹⁰⁺: 1313.2, [2M+11H]¹¹⁺: 1193.9, [2M+12H]¹²⁺: 1094.4, [2M+13H]¹³⁺: 1010.4, [2M+14H]¹⁴⁺: 938.3, [2M+15H]¹⁵⁺: 875.8, [2M+16H]¹⁶⁺: 821.1, [2M+17H]¹⁷⁺: 772.9, [2M+18H]¹⁸⁺: 730.0, [2M+19H]¹⁹⁺: 691.6, [2M+20H]²⁰⁺: 657.0 Mass Found (ESI+): 1874.9 [2M+7H]⁷⁺, 1640.6 [2M+8H]⁸⁺, 1458.2 [2M+9H]⁹⁺, 1312.8 [2M+10H]¹⁰⁺, 1193.6 [2M+11H]¹¹⁺, 1094.3 [2M+12H]¹²⁺, 1010.2 [2M+13H]¹³⁺, 938.0 [2M+14H]¹⁴⁺, 875.5 [2M+15H]¹⁵⁺, 820.9 [2M+16H]¹⁶⁺, 772.6 [2M+17H]¹⁷⁺, 729.9 [2M+18H]¹⁸⁺, 691.4 [2M+19H]¹⁹⁺, 656.8 [2M+20H]²⁰⁺.