Supplementary Figure 3: Gating strategy for isolating tumor-specific T cells. | Nature Protocols

Supplementary Figure 3: Gating strategy for isolating tumor-specific T cells.

From: RNase H–dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis

Supplementary Figure 3

The gating strategy for sorting T cells stimulated with autologous melanoma cells consisted of the following steps: (i) gating on single cells with lymphocyte physical parameter, (ii) gating on viable (Zombie Aqua negative) CD3+ CD4+ events, and (iii) gating on IL2-, TNFα-, and IFNγ-positive cells using unstimulated control sample to establish background signal. Single cells were sorted into plates using a 70-µm nozzle.

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