Supplementary Fig. 5: Example raw photometry traces.
From: Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators
a, Raw photometry signals demodulated from 490-nm (blue) and 405-nm (violet) channel. Notice photobleaching in both channels. b, Photometry signals after 405-nm signal is fitted to 490-nm signal by applying a least-squares fit. c, ΔF/F trace is calculated for each recording session as. (490-nm signal – fitted 405-nm signal)/fitted 405-nm signal. Notice this can effectively remove the contribution of photobleaching and potential contamination from motion artifacts. All procedures were performed in accordance with the guidelines of the National Institutes of Health and were approved by the Institutional Animal Care and Use Committee (IACUC) and by the Office of Laboratory Animal Resources at the California Institute of Technology.
