Extended Data Fig. 5: Time and clearing comparison in FLASH (reagent 1) and other methods. | Nature Protocols

Extended Data Fig. 5: Time and clearing comparison in FLASH (reagent 1) and other methods.

From: Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH

Extended Data Fig. 5: Time and clearing comparison in FLASH (reagent 1) and other methods.The alternative text for this image may have been generated using AI.

a, Comparison of processing time for each of the methods, from tissue collection to mounting for imaging (see also Extended Data Figs. 6–9). b, Images of tissues cleared with different methods. Yellow dotted lines indicate positions of tissue in completely transparent samples. AR: antigen retrieval; Per. or permeab.: permeabilisation; SWITCH: System-Wide control of Interaction Time and kinetics of CHemicals; CUBIC HV: Clear, Unobstructed Brain Imaging Cocktails and Computational analysis HistoVIsion; iDISCO: Immunolabeling-enabled three-Dimensional Imaging of Solvent-Cleared Organs.

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