Extended Data Fig. 2: Protocol optimization: enzymatic treatment applied at different steps of the procedure.
From: Isolation and characterization of extracellular vesicle subpopulations from tissues
a, The 2018 Protocol was used to isolate EVs without using collagenase D and DNase I (blue arrows). In the 2019 Protocol, collagenase and DNase I treatment was applied to the re-suspended vesicle-enriched pellets (green arrows). In the Final Protocol, collagenase D and DNase I treatment were applied directly to the tissue pieces after the dissociating step (red arrows). b and c, Micrograms of proteins isolated from lEVs (b) and sEVs (c) normalized to the grams of tissue for each protocol; 2018 Protocol, n = 4; 2019 Protocol, n = 2; Final Protocol 3, n = 13. Human samples were collected and processed in alignment with the Regional Ethical Review Board at the University of Gothenburg, Sweden (#096-12 and #995-16). The patient provided informed written consent. The image is modified from Crescitelli et al.35.
