Extended Data Fig. 1: Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2. | Nature Protocols

Extended Data Fig. 1: Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2.

From: Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

Extended Data Fig. 1: Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2.

Normalized expression of SLC4A4/NBCe1 and SLC5A2/SGLT2 (proximal tubule), SLC12A1/NKCC2 (loop of Henle), SLC12A3/NCC (distal tubule) and SCNN1A/ENaCα (collecting duct principal cells) in tubuloids in passages 4, 7 and 10. Tubuloids from two different donors were analyzed for all genes except SLC4A4/NBCe1 and SLC5A2/SGLT2 (both one donor). Expression was normalized to the housekeeping gene RPLP0. Mean and SEM are shown.

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