Supplementary Fig. 2: BN and SDS–PAGE of samples. | Nature Protocols

Supplementary Fig. 2: BN and SDS–PAGE of samples.

From: The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes

Supplementary Fig. 2: BN and SDS–PAGE of samples.

(a) BN-PAGE of E. coli membranes, without major outer membrane complexes, overexpressing the fap operon with and without DDM indicates the existence of two 180–200 kDa membrane protein complexes containing Fap proteins in the presence of DDM. (b) SDS–PAGE of C. crescentus membrane stalks containing the S-layer or extracted S-layer protein RsaA shows degradation products following low pH extraction.

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