Supplementary Fig. 3: Low m/z region of protein from the fap operon purified in detergent shows different FapD species dissociating from the FapF3–FapD complex.
From: The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes
High energy disruption of the FapF3–FapD complexes at 400 V induces their dissociation and reveals the co-existence of three different FapD species—a correctly processed FapD at 23,313 Da, an incompletely processed FapD’ at 24,596 Da and a self-processed FapD’’ with a mass of 22,488 Da. Spectrum is a representative from 2 biological repeats.
