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Guidance for quantitative confocal microscopy

Nature Protocols (2020)Cite this article

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This poster guides researchers through all aspects of acquiring quantitative confocal microscopy images. It provides recommendations from sample preparation to data acquisition and discusses common experimental and instrumentation pitfalls.

This poster is freely available online thanks to support from Carl Zeiss Microscopy GmbH.

The poster content is peer reviewed, editorially independent and the sole responsibility of Nature America, Inc., part of Springer Nature.

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  • 20 April 2020

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Authors and Affiliations

  1. Advanced Optical Microscopy Facility (AOMF), University Health Network, Toronto, Ontario, Canada

    James Jonkman

  2. Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada

    Claire M. Brown

  3. A*STAR Microscopy Platform (AMP), Skin Research Institute of Singapore, A*STAR, Singapore, Singapore

    Graham D. Wright

  4. Crick Advanced Light Microscopy Facility (CALM), The Francis Crick Institute, London, UK

    Kurt I. Anderson

  5. Bio-Imaging Resource Center, The Rockefeller University, New York, NY, USA

    Alison J. North

Authors
  1. James Jonkman
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  2. Claire M. Brown
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  3. Graham D. Wright
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  4. Kurt I. Anderson
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  5. Alison J. North
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Corresponding author

Correspondence to James Jonkman.

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Jonkman, J., Brown, C.M., Wright, G.D. et al. Guidance for quantitative confocal microscopy. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-0307-7

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  • DOI: https://doi.org/10.1038/s41596-020-0307-7

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