Extended Data Fig. 1: Sequence scheme of lentiMPRA. | Nature Protocols

Extended Data Fig. 1: Sequence scheme of lentiMPRA.

From: lentiMPRA and MPRAflow for high-throughput functional characterization of gene regulatory elements

Extended Data Fig. 1

a, Synthesized CRS oligo sequence. b, Primers and their binding in 1st and 2nd round PCR for library amplification. c, Recombination and plasmid library sequence. d, Primers and their binding in library amplification and sequencing for CRS–barcode association. e, Primers and their binding in reverse transcription, library amplification and sequencing for barcode counting.

Back to article page