Extended Data Fig. 3: FISH in combination with antibody staining of pectoral fin, skin and hair cells at 10 dpf. | Nature Protocols

Extended Data Fig. 3: FISH in combination with antibody staining of pectoral fin, skin and hair cells at 10 dpf.

From: Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae

Extended Data Fig. 3

a, The Tomato expresses in skin and hair cells at 10 dpf in the transgenic line Tg(krt18:Tomato). b, Labelling of FISH-ctgfa (a pectoral fin marker), anti-Tomato and DAPI in the pectoral fin (dashed line, top view) in Tg(krt18:Tomato) at 10 dpf (3D stack of images; 152 µm, 38 slices, 4 µm each slice; dorsal view). c, Labelling of FISH-ctgfa (a pectoral fin marker), anti-Tomato and DAPI in the pectoral fin (dashed line, front view) in Tg(krt18:Tomato) at 10 dpf (3D stack of images; 152 µm, 38 slices, 4 µm each slice; ventral view). Note that the Tomato expresses in the skin and FISH-ctgfa expresses in the pectoral fin under the skin. d, Labelling of FISH-krt18 (the skin and hair cells specific-gene), anti-Tomato and DAPI in skin and hair cells in Tg(krt18:Tomato) at 10 dpf (3D stack of images; 232 µm, 58 slices, 4 µm each slice). Note that the FISH-krt18 merges with Tomato and expresses strongly in hair cells (arrowheads) and weekly in the skin. BF, bright field. Scale bar, 100 µm.

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