Extended Data Fig. 1: FISH in combination with antibody staining of brain neuron and spinal cord at late embryonic and larval stage.

a, Labelling of FISH-huc (a gene specific to brain neurons and spinal cord), anti-GFP and DAPI in the whole body in the Tg(RasGFP) transgenic line at 4 dpf (3D stack of images; 448 µm, 32 slices, 14 µm each slice). b, Labelling of FISH-huc, anti-GFP and DAPI in the whole body in the Tg(RasGFP) transgenic line at 10 dpf (3D stack of images; 448 µm, 32 slices, 14 µm each slice). Note that huc mRNA is expressed in the brain neuron, spinal cord and intestine; the GFP and DAPI stained the whole body. Scale bar, 100 µm.