Extended Data Fig. 2: FISH in combination with antibody staining of notochord in larvae. | Nature Protocols

Extended Data Fig. 2: FISH in combination with antibody staining of notochord in larvae.

From: Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae

Extended Data Fig. 2

a, The Kaede expresses in the notochord at 5 and 10 dpf in the transgenic line Tg(ctgfa:Kaede). b, Labelling of FISH-ctgfa, anti-Kaede and DAPI in the notochord (dashed line) in Tg(ctgfa:Kaede) at 5 dpf (3D stack of images; 68 µm, 17 slices, 4 µm each slice). c, Labelling of FISH-ctgfa, anti-Kaede and DAPI in the notochord (dashed line) in Tg(ctgfa:Kaede) at 10 dpf (3D stack of images; 68 µm, 17 slices, 4 µm each slice). Scale bar, 100 µm.

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