Extended Data Fig. 2: Validation of vascular corrosion casting in postnatal (P10) and adult (P60) mice using light microscopy (LM) and SEM.

a-f Quality control of the vascular corrosion casts of P10 and P60 WT mice by visual inspection by LM (a), and by SEM (b-f). a Light microscopy image of the entire brain vasculature of the P60 WT mice (gold sputtered for SEM). b SEM image of the entire brain vascular corrosion cast illustrating the dense vascular network including blood vessels of all sizes with recognizable vascular anatomy. c-f The entire brain was uniformly filled with resin and the lumen of the vessels imprinted in the casting material with the finest details. e The capillary network in the cortex is well defined and devoid of interruptions. f Note the elongated cellular and nuclear imprints on the large vessel that are also typical for good-quality vascular corrosion casts in adult mice¹⁰⁵. Scale bars, 4 mm (a); 4 mm (b); 50 μm (c); 50 μm (d), 100 μm (e), 100 μm (f). a,d-f reproduced with permission from ref. 39. b reproduced with permission from ref. ¹⁰⁰.