Extended Data Fig. 3: Sequential 32-channel lambda-scan performed on a Leica SP8.
From: Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D
a, Optical section of unmixed eight-color labeled human embryonic kidney. DAPI (not shown), KI67 (aqua), PAX8 (yellow), NCAM1 (magenta), SIX2 (green), CDH1 (red), CDH6 blue) and F-ACTIN (gray). Scale bar, 50 μm. b, Magnification of white inset of a depicting optical sections of combinations of overlay channels for visual inspection. DAPI (gray), F-ACTIN (signal intensity gradient: red-yellow-white) (left), CDH1 (blue), SIX2 (green), NCAM1 (red) (middle), CDH6 (blue), KI67 (green), PAX8 (red) (right). Scale bars, 50 μm. c, Single-channel optical sections of the white inset in a for visual inspection. Scale bars, 50 μm.
