Extended Data Fig. 1: Graphic scheme of the trapping nano-LC system. | Nature Protocols

Extended Data Fig. 1: Graphic scheme of the trapping nano-LC system.

From: High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

Extended Data Fig. 1

A large-i.d. trapping column (300 μm i.d.) and 65-cm-long, 75 μm i.d. C18 separation column, heated at 50 °C, are connected and operated by a six-port valve. The unique selective trapping-delivery strategy consists of three stages, including sample injection, cleanup and delivery. The red lines and arrows indicate the flow of the peptide sample in each stage.

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