Extended Data Fig. 4: Assessment of permeabilization timing and plate type on Cell Painting performance.
From: Optimizing the Cell Painting assay for image-based profiling

a, Performing permeabilization at the same time as staining produces comparable percent replicating and percent matching results. b, PhenoPlates without barrier wells and Aurora plates with barrier wells produce comparable percent replicating and percent matching results. For more information including the plate(s) represented by each data point, see the Source Data file for this figure; expanded experimental details for each plate may be found in Supplementary Data File 1.