Extended Data Fig. 6: Further assessment of imaging conditions on Cell Painting performance.
From: Optimizing the Cell Painting assay for image-based profiling
a, Imaging of the same plates on a wide-field microscope with 2 × 2 binning versus a different manufacturer’s microscope in confocal mode with 1 × 1 binning. No major differences are observed. b, Acquisition of multiple Z planes slightly improves percent replicating but not percent matching in two batches. For more information including the plate(s) represented by each data point, see the Source Data file for this figure; expanded experimental details for each plate may be found in Supplementary Data File 1.
