Extended Data Fig. 8: Change in Cell Painting performance when adding individual channel features.

Change in mean percent replicating of eight JUMP-MOA plates stained with the final staining conditions when an individual stain is present versus not; each chart shows for a particular stain when added to the non-channel-specific features plus zero or more other stains (x axis) the change in the mean percent replicating (y axis) when those features are included. The color(s) of each marker indicate which channel(s) are already present. Note that these results are not the same as truly having the stains not present, because (i) a channel still may have been used in creating the initial segmentation results, and (ii) it does not account for cross-talk between stains. The absolute percent replicating numbers are available in Extended Data Fig. 7. For more information including the plates represented in this figure, see the Source Data file for this figure; expanded experimental details for each plate may be found in Supplementary Data File 1.