Extended Data Fig. 5: Two methods for baseline correction.

For each growth curve, two baseline correction methods are applied. This is illustrated here by using three examples (one per row). In the first method (second column), a constant shift is subtracted from all time points of the growth curve, setting the minimum value to zero. In the second method (third column), an initial perturbation that affects earlier times more than later times (e.g., due to condensation) is assumed; a constant shift is subtracted as described earlier. The curve is then rescaled so that an uncorrected OD value of 1 would also have a corrected OD value of 1. AUCs are calculated for both scenarios, with reference compounds used to set the AUC to 1 after rescaling. The baseline correction that resulted in an AUC closest to 1, indicative of normal growth (conservative approach), is selected for each compound (highlighted in bold). Note that the figure is presented as a simplified illustrative example and that the curves depicted do not correspond to actual bacterial growth curves.