Extended Data Fig. 6: Additional information for Fig. 3.
From: Production and use of antigen tetramers to study antigen-specific B cells
a) Evaluation of phenotype of OVA-specific switched and IgE-expressing B cells from OVA/Alum immunized Verigem mice at 7 days post-immunization. b) Concatenated plot of intracellular IgE staining from 3 mice sensitized by 4 gavages of OVA/CT. Data was collected at day 6 after the last gavage. c) Extracellular IgG1 staining of OVA tetramer-binding B cells from unimmunized and OVA/Alum immunized mice. d) Intracellular IgG1 staining with and without blocking surface IgG1 with an unlabeled antibody.
