Extended Data Fig. 2: Antigen-specific B cell tracing by adoptive transfer of tetramer enriched cells.
From: Production and use of antigen tetramers to study antigen-specific B cells
a) Schematic of the experiment. b) Representative plots and quantification of control and OVA tetramer staining of spleen and mesenteric lymph nodes of recipient µMT mice 9 days after OVA/Alum immunization. c) Quantification of serum OVA-specific IgE and IgG1 antibodies by ELISA. d–f) Evaluation of phenotype of OVA-specific B-lineage cells in the enriched fraction of OVA/Alum-immunized µMT recipients. * p<0.05 by two-way ANOVA. Error bars represent SEM. Panel a created with BioRender.com.
