Extended Data Fig. 7: Scanner setup instructions.
From: Simple large-scale quantitative phenotyping and antimicrobial susceptibility testing with Q-PHAST

This figure illustrates the assembly steps for establishing the system outlined in the protocol. a, Unpacked Epson V600 photo scanner. b, The scanner with the lid removed and positioned at the bottom, leaving the scanner surface exposed. For ease of handling, it can be secured with black adhesive tape. c, A template indicating the positions of the plates on the scanner is presented. With the front buttons on the left, the first plate corresponds to the upper left, the second to the upper right, the third to the lower left and the fourth to the lower right. d, By using the template from c, an EVA foam template is cut to the dimensions of the omnitray plates. The cut sections serve as opaque covers for each plate. e, The template from the previous step is placed on the scanning surface to enhance plate placement, keeping them fixed and immobile. The non-plate area remains entirely black, improving image capture. f, Three scanners, prepared as described previously, are arranged inside a temperature-controlled incubator, with cable connections accessible externally. g, Final assembly of three scanners within a temperature-controlled incubator. At the top is the computer that controls and stores images from the scanners. The scanners connect to this computer via USB. Both cables and scanners are labeled with colors for traceability. Scanner power cables are organized on the opposite side for orderly connection to the power supply. The surfaces of the incubator exposed to light are covered to ensure total darkness when the incubator is closed, thereby improving image quality and homogeneity.